g , Relative fold change in cell number of the indicated HEK-293T SLC25A39 knockout cells expressing a vector control or SLC25A39 cDNA transduced with the indicated sgRNAs
The mechanism is well-characterized at the biochemical level: GSH inhibits tyrosinase, the rate-limiting enzyme in melanin biosynthesis, by binding to its copper-containing active site
Malondialdehyde (MDA) and O 2 production were significantly increased by 60.6% and 79.9%, respectively, under the HT treatment, and by 23.2% and 30.2%, respectively, under the HT+GSH treatment, compared to the NT treatment (Fig
Overall, these findings indicate that although intracellular GSH does not affect tumour growth and survival, extracellular GSH is highly abundant and potentially supports tumour growth
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